Everything you need to know about day two of Royal Ascot on Wednesday

Big races

The Prince of Wales's Stakes (4.20) headlines day two of the Royal meeting as the only Group 1 race on the card.

Run over 1m2f, it is the most prestigious race of the year for middle- distance horses aged four and above.

The 1m6f Group 2 Queen's Vase (3.05) provides a potentially valuable insight into future staying stars, with three-time Gold Cup winner Stradivarius having been successful in 2017.

The Royal Hunt Cup (5.00) is as highly competitive as ever and presents one of the trickiest betting handicaps of the season with 30 runners set to charge down the straight mile.

Big horses

Love takes on the boys for the first time in the Prince of Wales's Stakes for Aidan O'Brien. The dual Classic-winning filly makes her eagerly anticipated reappearance for Ryan Moore on her first trip to Ascot.

Dual Classic winner Love reappears in the feature Group 1 Prince of Wales's StakesCredit: Edward Whitaker

Lady Bowthorpe, who took her form to new heights when second to Palace Pier in last month's Lockinge, will bid to go one place better in the Group 2 Duke of Cambridge Stakes for older fillies and mares.

Among the opposition is Group 1 winner Champers Elysees, wide-margin York scorer Queen Power and last year's Sandringham winner Onassis.

Ward aims for repeat in the opener

Wesley Ward's sole winner at the meeting last year came in the opening 5f sprint for juvenile fillies, the Queen Mary Stakes (2.30), with Campanelle for owners Stonestreet Stables.

The same connections hold a strong chance of a repeat this year, with favourite Twilight Gleaming aiming to give the 53-year-old American a 12th Royal Ascot winner.

Wesley Ward and Stonestreet Stables will be aiming to repeat Campenelle's (left) success in the Queen Mary Stakes with Twilight GleamingCredit: Edward Whitaker

Stay ahead of the field with the Racing Post

The Racing Post has you covered every day of Royal Ascot. From the morning, with a bumper Racing Post newspaper available to purchase from all good newsagents, throughout the afternoon, with host Dave Orton, Bruce Millington and the might of the racing world guiding you through the action on Racing Post Live, through to the evening, with leading Racing Post tipsters Tom Segal and Paul Kealy joining Ross Brierley on our live show, In The Know (7-8pm, Monday to Friday).

You can download the following day's Racing Post from 9pm the night before. Subscribe to Members' Club Ultimate for unlimited access to the Racing Post newspaper, online and via the app, saving more than £100 a month on the cover price. Find out more here.

You can also keep up-to-date with the latest news, previews, reports and tips on racingpost.com and on the Racing Post mobile app every day during what promises to be an awesome Royal Ascot.

Wednesday
2-6.20pm: Racing Post Live with the Betfair Exchange
7-8pm: In The Know in association with Coral

Thursday
2-6.20pm: Racing Post Live with the Betfair Exchange
7-8pm: In The Know in association with Coral

Friday
2-6.20pm: Racing Post Live with the Betfair Exchange
7-8pm: In The Know in association with Coral

Plus What A Shout and the Racing Postcast

Saturday
2-6.20pm: Racing Post Live with the Betfair Exchange

All live shows are available to watch via YouTube and Facebook, with the Postcasts available via the usual podcast platforms

Read more on Royal Ascot 2021:

Royal Ascot tips: Tom Segal, Paul Kealy and Tom Collins share their best bets

The Punting Club Royal Ascot special: 'She's my best – and only – bet in a two-year-old race'

Confirmed runners and riders for the Prince of Wales's Stakes at Royal Ascot

Check out the Racing Post Royal Ascot microsite for the latest tips and odds for the royal meeting. The best Royal Ascot betting advice and exclusive free betting offers all in one place.